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Clinical Chemistry 18: 654-657, 1972;
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Clinical Chemistry, Vol 18, 654-657, Copyright © 1972 by the American Association for Clinical Chemistry

Assay of Human Plasminogen in Plasma by Affinity Chromatography

Raymond P. Zolton 1, Edwin T. Mertz 1, and Henry T. Russell 1

1 Department of Biochemistry, Purdue University, Lafayette, Ind. 47907.

A direct, rapid (<3h), and simple technique is described for assaying human plasminogen by affinity chromatography. Plasma is passed over a column of Sepharose—butyl aminobenzoate. The unretarded proteins, including plasmin, are washed out with phosphate—saline, pH 7.4, buffer. The bound plasminogen is released by adding lysine, 0.1 mol/liter, to the buffer and the plasminogen concentration in the eluate is determined by absorbance measurements at 280 nm. A value of 195 ± 10 mg/liter was found for pooled donor plasma from normal individuals. Twenty-one normal women, age 21 to 46, had an average value of 194 ± 38 mg/liter, while 14 pregnant women in the third trimester had an average value of 250 ± 55 mg/liter. The range in 30 hospital patients with various ailments was 122 to 265 mg/liter.


Key Words: normal values • values in hospital patients, late pregnancy • affinity chromatography on Sepharose-butyl aminobenzoate

Submitted on March 31, 1972
Accepted on April 27, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.