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Clinical Chemistry 18: 673-674, 1972;
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Clinical Chemistry, Vol 18, 673-674, Copyright © 1972 by the American Association for Clinical Chemistry

Improved Determination of Total Serum Lipids by the Sulfo-Phospho-Vanillin Reaction

Christopher S. Frings 1, Ted W. Fendley 1, Ralph T. Dunn 1, and Cecelia A. Queen 1

1 Medical Laboratory Associates, 1025 S. 18th St., Birmingham. Ala. 35205.

We have modified and improved a colorimetric method for determining total serum lipids [Amer. J. Clin. Pathol. 53, 89 (1970)]. The improved procedure requires only 20 µl of serum, and the entire determination is done in one tube, thus eliminating the tedious pipetting of a concentrated sulfuric acid-serum digest. The phospho-vanillin reagent has been modified so that the linearity of the method now extends to 1250 mg/dl. Concentrations up to 1240 mg/dl or of bilirubin up to 20 mg/dl in the serum do not interfere. The relative standard deviation for the method (day-to-day) is 4.9%.


Key Words: sulfo-phospho-vanillin reaction • olive oil as a standard

Submitted on March 24, 1972
Accepted on April 28, 1972




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