Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 675-676, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dill, W. A.
Right arrow Articles by Glazko, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dill, W. A.
Right arrow Articles by Glazko, A. J.

Clinical Chemistry, Vol 18, 675-676, Copyright © 1972 by the American Association for Clinical Chemistry

Fluorometric Assay of Diphenylhydantoin in Plasma or Whole Blood

Wesley A. Dill 1 and Anthony J. Glazko 1

1 Department of Pharmacology, Research and Development Division, Parke, Davis & Co., Ann Arbor, Mich. 48106.

A highly sensitive assay procedure for 5,5-diphenylhydantoin (DPH) involves oxidation with alkaline permanganate to form benzophenone. This is then extracted with heptane, allowed to react with concentrated sulfuric acid, and the fluorescence of the reaction product is measured. The procedure appears to be fairly specific for DPH; 5-(p-hydroxyphenyl)-5-phenylhydantoin (a DPH metabolite), phenobarbital, or other anticonvulsant drugs do not interfere. The sensitivity of the fluorometric procedure permits assays on finger-blood specimens. It provides a simple means for evaluating the effectiveness of a given dose schedule in different subjects.

Submitted on April 7, 1972
Accepted on May 10, 1972






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.