Clinical Chemistry
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Clinical Chemistry 18: 749-752, 1972;
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Clinical Chemistry, Vol 18, 749-752, Copyright © 1972 by the American Association for Clinical Chemistry

Dynamic Introduction of Whole-Blood Samples into Fast Analyzers

Charles D. Scott 1 and James C. Mailen 1

1 Oak Ridge National Laboratory,2 Oak Ridge, Tenn. 37830.

A new automated method of sample preparation and introduction that may circumvent the problems of measurement of micro-scale quantities of samples and preparation of serum or soluble blood fraction is being developed for use in fast analytical systems based on the GeMSAEC principle. This method involves the dynamic introduction of reagents or blood samples into the centrifugal field of the Fast Analyzer during rotation of the rotor. The rotor can be designed to fractionate the fluid into discrete, measured portions that are transferred to the cuvets for photometric monitoring. Whole-blood samples can be directly introduced into the rotating rotor where, after fractionation into measured volumes, the particulates are automatically removed by the centrifugal field and the resulting measured quantities of plasma are transferred directly to the cuvets. The dynamic proportionation of reagents, serum, and whole blood has been shown to be precise, and automatic analyses for specific chemicals have been performed on whole blood samples with a coefficient of variation of less than 2%.




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C. D. Scott
Health Care Delivery and Advanced Technology
Science, June 29, 1973; 180(4093): 1339 - 1342.
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Copyright © 1972 by the American Association for Clinical Chemistry.