Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 783-788, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saunders, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saunders, A. M.

Clinical Chemistry, Vol 18, 783-788, Copyright © 1972 by the American Association for Clinical Chemistry

Development of Automation of Differential Leukocyte Counts by Use of Cytochemistry

Alex M. Saunders 1

1 The Technicon Instruments Corp., 511 Benedict Ave., Tarrytown, N. Y. 10591; and New York Medical College, Flower & Fifth Ave., New York, N. Y. 10029.

Development of automation in cytochemistry is reviewed. A variety of disciplines had to be merged to accomplish the following. Samples of anticoagulated whole blood are processed chemically in a continuous-flow system having precise proportioning of reagents and exact time sequences for reactions. While red blood cells are lysed, color contrast is developed in specific white blood cells. These are counted and differentiated by their light absorption and light scattering characteristics, typically at a rate of 500-2000 per second, in an automatic electro-optical device through which the cell suspension flows. The procedure is highly reproducible because it relies on physical constants rather than human judgment and because greater replication of sample is possible in the same time.


Key Words: peroxidase in human leukocytes • continous-flow cytochemistry • optical sensing • signal processing • 4-chloro-1-naphthol as chromogen • pulse-height analysis curves • Hemalog D




The following articles in journals at HighWire Press have cited this article:


Home page
 ThoraxHome page
M. J Leckie, S. A Bryan, J. Khan, A. Dewar, S. L Aikman, J. McGrath, D. A Okrongly, J. F Burman, P. J Barnes, and T. T Hansel
Automated quantitation of circulating neutrophil and eosinophil activation in asthmatic patients
Thorax, June 1, 2000; 55(6): 471 - 477.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.