Clinical Chemistry
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Clinical Chemistry 18: 829-840, 1972;
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Clinical Chemistry, Vol 18, 829-840, Copyright © 1972 by the American Association for Clinical Chemistry

Enzymatic Kinetic Rate and End-Point Analyses of Substrate, by Use of a GeMSAEC Fast Analyzer

T. O. Tiffany 1, J. M. Jansen 1, C. A. Burtis 1, J. B. Overton 1, and C. D. Scott 1

1 Molecular Anatomy (MAN) Program,1 Oak Ridge National Laboratory,2 Oak Ridge, Tenn. 37830.

Enzymatic substrate analysis is an attractive means of analysis in clinical chemistry because of its sensitivity and specificity. The GeMSAEC Fast Analyzer, in conjunction with a small computer, provides a means of performing routine enzymatic substrate analysis and offers the following advantages: (a) selectivity of approaches to enzymatic analysis, i.e., end-point or kinetic; (b) essentially parallel analyses of multiple samples, yielding a unique method for performing kinetic fixed-time analysis; (c) on-line data reduction, resulting in rapid calculation and output of results and the minimization of data handling errors; and (d) a small reagent volume per test (400 µl), which reduces the cost of analysis. The analysis of substrate with enzymatic end-point and kinetic procedures is examined by use of a computer-interfaced Fast Analyzer. Computer programs were written to facilitate this study. Glucose (hexokinase/GPD), urea (urease/GMD), and uric acid (uricase) have been used as examples in evaluating both end-point and kinetic analyses. The advantages and limitations of each type of analysis are presented, with the emphasis being placed on enzymatic substrate analysis and means by which the computer-interfaced Fast Analyzer can facilitate both end-point and kinetic analyses.


Key Words: hexokinase • urease • uricase • equilibrium analysis • FOCAL • glucose • urea • uric acid • rate or fixed-time analyses




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Copyright © 1972 by the American Association for Clinical Chemistry.