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Clinical Chemistry 18: 841-844, 1972;
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Clinical Chemistry, Vol 18, 841-844, Copyright © 1972 by the American Association for Clinical Chemistry

Kinetic Method for Determining Acid Phosphatase Activity in Serum with Use of the "CentrifiChem"

Diane L. Fabiny-Byrd 1 and Gerhard Ertingshausen 1

1 Union Carbide Research Institute, Tarrytown, N. Y. 10591.

Acid phosphatase activity is determined by splitting 1-naphthyl phosphate, concurrently diazotizing the released 1-naphthol with Fast Red TR, and measuring the resulting color. The test is performed in the presence and absence of tartrate. Reaction rates can be continuously monitored, and their difference is proportional to acid phosphatase activity that is inhibited by tartrate. Results for sera with normal and increased acid phosphatase activities are presented and three different methods for acid phosphatase are compared. The kinetic blank used in the reaction eliminates all nonenzymatic contributions to substrate splitting.






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Copyright © 1972 by the American Association for Clinical Chemistry.