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Clinical Chemistry 18: 1001-1004, 1972;
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Clinical Chemistry, Vol 18, 1001-1004, Copyright © 1972 by the American Association for Clinical Chemistry

Modified Automated Determination of 2,3-Diphosphoglycerate in Whole Blood

Kenneth F. Atkinson 1

1 Pulmonary Research Laboratories, Veterans Administration Hospital, Indianapolis, Ind. 46202.

A modification is described of the automated determination of 2,3-diphosphoglycerate (DPG) in blood [Grisolia, S., et al., Anal. Biochem. 31, 235 (1969)]. Modifications in the manifold result in a sensitive, noise-free, rapid system and the modifications in the preparations of the reagents ensure stability of the diluted standards and blood samples for at least three weeks. Samples are run at the rate of 60/h and sample size can be as small as 5 µl of whole blood. The coefficient of variation of the overall determination of automated DPG and manual hemoglobin is 3.6% and the SD is ±0.77 µmol/g Hb. The normal range is 14.6 ± 2.2 (SD) µmol/g hemoglobin.


Key Words: AutoAnalyzer • commercial enzymes • normal values

Submitted on April 24, 1972
Accepted on July 7, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.