Clinical Chemistry
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Clinical Chemistry 18: 1009-1012, 1972;
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Clinical Chemistry, Vol 18, 1009-1012, Copyright © 1972 by the American Association for Clinical Chemistry

An Error-Producing Interaction in an Automated Method for Measuring Cholinesterase Activity in Blood

Robert I. Ellin 1, William A. Groff 1, and Andris Kaminskis 1

1 Clinical Laboratory Section, Clinical Research Branch, Medical Research Division, Biomedical Laboratory, Edgewood Arsenal, Md. 21010.

Blood cholinesterase activity values obtained in our laboratory by the automated procedure of Levine et al. (Automat. Anal. Chem., Technicon Symp. 1965) are greater than those values obtained by a comparable manual method. This problem is caused by reaction of the standard (reduced glutathione) with the substrate (acetylthiocholine) to form acetylglutathione and thiocholine. Because thiocholine dialyzes about three times more rapidly than glutathione in the automated system and gives the same color reaction, proportionately higher activity values would be reported. Ways of correcting this problem are presented.


Key Words: dialysis in automated methods • thin-layer chromatography • glutathione reaction with acetylthiocholine • AutoAnalyzer • dialysis in an automated system • thiocholine standard

Submitted on December 29, 1971
Accepted on July 7, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.