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Clinical Chemistry 18: 1019-1021, 1972;
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Clinical Chemistry, Vol 18, 1019-1021, Copyright © 1972 by the American Association for Clinical Chemistry

Simplified Rapid Electroimmunodiffusion Technique for Measuring agr1-Antitrypsin in Serum

Ernest R. Manildi 1

1 Medical Service and Respiratory Care Unit, Veterans Administration Hospital, Spokane, Wash. 99208.

A rapid method is described for immunochemical quantitation of agr1-antitrypsin by electroimmunodiffusion on cellulose acetate. Compared to earlier qualitative screening techniques for trypsin activity, including trypsin inhibitory capacity, the procedure presented here is specific, reproducible, and quantitative for agr1-antitrypsin. It is inexpensive and less time consuming than electroimmunodiffusion or radial immunodiffusion in agar gel. The test is so simply done that this procedure can be routinely used by most hospital clinical laboratories. The technique is easily adapted to immunochemical quantitation of other protein fractions.


Key Words: normal values, men • degenerative lung disease

Submitted on April 27, 1972
Accepted on July 5, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.