Clinical Chemistry
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Clinical Chemistry 18: 928-933, 1972;
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Clinical Chemistry, Vol 18, 928-933, Copyright © 1972 by the American Association for Clinical Chemistry

Multichannel Analyzer Featuring Ultrahigh Stability Photometry: Application to Enzyme Determination

Harry L. Pardue 1 and Michael M. Miller 1

1 Department of Chemistry, Purdue University, Lafayette, Ind. 47907.

An instrument system has been developed for the simultaneous photometric monitoring of multiple sample channels. Salient features of the system include ultrahigh photometric stability, random access to individual channels, electronic channel selection, wavelength selectivity among the different channels, and either manual or computercontrolled operation. The system implemented in this work consists of 10 sample channels arranged radially around a single stabilized source. It has been evaluated for the kinetic determination of alkaline phosphatase in reconstituted sera. The drift of the photometer averages less than 0.03% T (1.3 x 10-4 A) per hour. Assay values for alkaline phosphatase exhibit standard deviations of 2% or less in most cases.


Key Words: alkaline phosphatase • automated enzyme activity determination • multichannel analytical systems • parallel kinetic measurements • computerization • high-stability photometer • GeMSAEC compared

Submitted on May 3, 1972
Accepted on June 5, 1972







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Copyright © 1972 by the American Association for Clinical Chemistry.