Clinical Chemistry
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Clinical Chemistry 18: 951-955, 1972;
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Clinical Chemistry, Vol 18, 951-955, Copyright © 1972 by the American Association for Clinical Chemistry

Isolation of 2-Acetamido-1-beta-(L-beta-Aspartamido)-1,2-Dideoxy-D-Glucose from Normal Human Urine

Barbara O’Neill Rowley 1 and Paul B. Hamilton 1

1 Department of Biochemistry, Alfred I. du Pont Institute, Wilmington, Del. 19899.

A glycopeptide was isolated from normal human urine by fractionation on a column of Sephadex G-10 and preparative ion-exchange chromatography. Elution behavior during ion-exchange chromatography in two different solvent systems, amino acids formed upon hydrolysis, and migration on high-voltage electrophoresis and thin-layer chromatography were essentially identical for this substance and for authentic 2-acetamido-l-beta-(L-beta-aspartamido)-1,2-dideoxy-D-glucose. A technique was developed to permit analytical-scale fractionation of individual urines followed by analysis for this glycopeptide; urine from two normal individuals contained 7 and 11 µmol of 2-acetamido-1-beta-(L-beta-aspartamido)-1,2-dideoxy-D-glucose per liter.


Key Words: urinary glycopeptides • mental retardation • normal values • cation-exchange chromatography • high-voltage electrophoresis • thin-layer chromatography • amino acid composition

Submitted on June 7, 1972
Accepted on June 14, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.