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Clinical Chemistry 18: 965-967, 1972;
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Clinical Chemistry, Vol 18, 965-967, Copyright © 1972 by the American Association for Clinical Chemistry

Automated Vitamin C Analysis

Hans U. Aeschbacher 1 and R. Glenn Brown 1

1 Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada.

An automated method of analysis for vitamin C (ascorbic acid, dehydroascorbic acid, diketogulonic acid) is described, in which ascorbic acid as oxidized to dehydroascorbic acid with Chloramine-T and determined by reaction with 2,4-dinitrophenylhydrazine. Results obtained by automated and manual methods agreed within 0.89% for ascorbic acid in liver. By the automated method, 96.7% of ascorbic acid added to tissue homogenates could be accounted for. The principal drawback to the proposed method is the use of 70% perchloric acid, a potent oxidizing agent, to solubilize the osazone.


Key Words: 2,4-dinitrophenylhydrazine • AutoAnalyzer • ascorbic acid, dehydroascorbic acid, diketogulonic acid • perchloric acid oxidant • Chloramine-T

Submitted on January 31, 1972
Accepted on June 15, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.