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Clinical Chemistry, Vol 18, 965-967, Copyright © 1972 by the American Association for Clinical Chemistry
1 Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada.
An automated method of analysis for vitamin C (ascorbic acid, dehydroascorbic acid, diketogulonic acid) is described, in which ascorbic acid as oxidized to dehydroascorbic acid with Chloramine-T and determined by reaction with 2,4-dinitrophenylhydrazine. Results obtained by automated and manual methods agreed within 0.89% for ascorbic acid in liver. By the automated method, 96.7% of ascorbic acid added to tissue homogenates could be accounted for. The principal drawback to the proposed method is the use of 70% perchloric acid, a potent oxidizing agent, to solubilize the osazone.
Submitted on January 31, 1972
Accepted on June 15, 1972
The following articles in journals at HighWire Press have cited this article:
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J. Z. Miller, W. E. Nance, J. A. Norton, R. L. Wolen, R. S. Griffith, and R. J. Rose Therapeutic Effect of Vitamin C: A Co-Twin Control Study JAMA, January 17, 1977; 237(3): 248 - 251. [Abstract] [PDF] |
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