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Clinical Chemistry 18: 968-970, 1972;
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Clinical Chemistry, Vol 18, 968-970, Copyright © 1972 by the American Association for Clinical Chemistry

Comparison of Ascorbic Acid Concentrations in Whole Blood Obtained by Venipuncture or by Finger Prick

Daniel W. Bradley 1, James E. Maynard 1, and Gladys Emery 1

1 Center for Disease Control, Phoenix Laboratories, 4402 North 7th St., Phoenix, Ariz. 85014.

Vitamin C (ascorbic acid) concentrations in whole blood obtained by finger prick or by venipuncture have been shown to be equivalent when measured by the 2,4-dinitrophenylhydrazine procedure. Whole blood obtained by either technique is stable for at least 6 h at 25°C, and for as long as 28 days when stabilized with trichloroacetic acid and stored at 4°C. Storage of whole blood at -70°C is accompanied by an initial loss (10-20%) of vitamin C; however, additional losses of vitamin C do not occur beyond the third day of storage.


Key Words: 2,4-dinitrophenylhydrazine procedure • vitamin C • stability on storage

Submitted on April 6, 1972
Accepted on July 3, 1972




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Copyright © 1972 by the American Association for Clinical Chemistry.