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Clinical Chemistry, Vol 18, 980-983, Copyright © 1972 by the American Association for Clinical Chemistry
-Hydroxylase
Activity in Human Blood
1 Roche Institute of Molecular Biology, Nutley, N.J.
07110.
We describe a simple, rapid, and sensitive procedure for measuring dopamine-
-hydroxylase
activity in human blood. The assay is based on the
enzymatic conversion of tyramine to octopamine
[1-(p-hydroxyphenyl)-2-aminoethanol], which is
then oxidized to p-hydroxybenzaldehyde and determined photometrically. Activities are assayed
on 2-20 µl of human serum or plasma obtained
from fingertip blood samples. The method should
prove useful in clinical studies, including the field
of pediatrics.
Submitted on May 19, 1972
Accepted on June 26, 1972
The following articles in journals at HighWire Press have cited this article:
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  E. M. Garland, B. K. Black, P. A. Harris, and D. Robertson Dopamine-beta-hydroxylase in postural tachycardia syndrome Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H684 - H690. [Abstract] [Full Text] [PDF]
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 R. Rej Clinical Chemistry through Clinical Chemistry: A Journal Timeline Clin. Chem., December 1, 2004; 50(12): 2415 - 2458. [Abstract] [Full Text] [PDF]
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R. Rej They Use Enzymes for Everything! Clin. Chem., June 1, 1998; 44(6): 1149 - 1153. [Full Text] [PDF]
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 A. K. Shapiro, M. Baron, E. Shapiro, and M. Levitt Enzyme Activity in Tourette's Syndrome Arch Neurol, March 1, 1984; 41(3): 282 - 285. [Abstract] [PDF]
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 J. L. Sullivan, C. N. Stanfield, S. Schanberg, and J. Cavenar Platelet Monoamine Oxidase and Serum Dopamine-{beta}-Hydroxylase Activity in Chronic Alcoholics Arch Gen Psychiatry, October 1, 1978; 35(10): 1209 - 1212. [Abstract] [PDF]
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 R. H. McDonald Jr, C. N. Corder, A. H. Vagnucci, and J. Shuman The Multiple Factors Affecting Plasma Renin Activity in Essential Hypertension Arch Intern Med, April 1, 1978; 138(4): 557 - 561. [Abstract] [PDF]
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F. Gotoh, T. Kanda, F. Sakai, M. Yamamoto, and T. Takeoka Serum Dopamine-{beta}-Hydroxylase Activity in Migraine Arch Neurol, September 1, 1976; 33(9): 656 - 657. [Abstract] [PDF]
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 I. J. KOPIN, S. KAUFMAN, H. VIVEROS, D. JACOBOWITZ, C. R. LAKE, M. G. ZIEGLER, W. LOVENBERG, and F. K. GOODWIN Dopamine- -Hydroxylase: Basic and Clinical Studies Ann Intern Med, August 1, 1976; 85(2): 211 - 223. [Abstract] [PDF]
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 T Nagatsu, K Ikuta, Y Numata, T Kato, and M Sano Vascular and brain dopamine beta-hydroxylase activity in young spontaneously hypertensive rats Science, January 23, 1976; 191(4224): 290 - 291. [Abstract] [PDF]
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F. Okada, I. Yamashita, and N. Suwa Two Cases of Acute Pandysautonomia Arch Neurol, March 1, 1975; 32(3): 146 - 151. [Abstract] [PDF]
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S. Rockson, R. Stone, M. Van Der Weyden, and W. N. Kelley Lesch-Nyhan Syndrome: Evidence for Abnormal Adrenergic Function Science, December 6, 1974; 186(4167): 934 - 935. [Abstract] [PDF]
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S. M. Schanberg, R. A. Stone, N. Kirshner, J. C. Gunnells, and R. R. Robinson Plasma Dopamine beta-Hydroxylase: A Possible Aid in the Study and Evaluation of Hypertension Science, February 8, 1974; 183(4124): 523 - 525. [Abstract] [PDF]
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