Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 18: 980-983, 1972;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagatsu, T.
Right arrow Articles by Udenfriend, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagatsu, T.
Right arrow Articles by Udenfriend, S.

Clinical Chemistry, Vol 18, 980-983, Copyright © 1972 by the American Association for Clinical Chemistry

Photometric Assay of Dopamine-beta-Hydroxylase Activity in Human Blood

Toshiharu Nagatsu 1 and Sidney Udenfriend 1

1 Roche Institute of Molecular Biology, Nutley, N.J. 07110.

We describe a simple, rapid, and sensitive procedure for measuring dopamine-beta-hydroxylase activity in human blood. The assay is based on the enzymatic conversion of tyramine to octopamine [1-(p-hydroxyphenyl)-2-aminoethanol], which is then oxidized to p-hydroxybenzaldehyde and determined photometrically. Activities are assayed on 2-20 µl of human serum or plasma obtained from fingertip blood samples. The method should prove useful in clinical studies, including the field of pediatrics.


Key Words: familial dysautonomia • idiopathic orthostatic hypotension • infantile hypoglycemia • hypertension • pediatric analyses • diagnostic aid • inhibitors in urine • normal values • norepinephrine • sympathetic nervous system • tyramine • octopamine

Submitted on May 19, 1972
Accepted on June 26, 1972




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. M. Garland, B. K. Black, P. A. Harris, and D. Robertson
Dopamine-beta-hydroxylase in postural tachycardia syndrome
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H684 - H690.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Rej
They Use Enzymes for Everything!
Clin. Chem., June 1, 1998; 44(6): 1149 - 1153.
[Full Text] [PDF]

Home page
 ScienceHome page
T Nagatsu, K Ikuta, Y Numata, T Kato, and M Sano
Vascular and brain dopamine beta-hydroxylase activity in young spontaneously hypertensive rats
Science, January 23, 1976; 191(4224): 290 - 291.
[Abstract] [PDF]

Home page
 ScienceHome page
S. Rockson, R. Stone, M. Van Der Weyden, and W. N. Kelley
Lesch-Nyhan Syndrome: Evidence for Abnormal Adrenergic Function
Science, December 6, 1974; 186(4167): 934 - 935.
[Abstract] [PDF]

Home page
 ScienceHome page
S. M. Schanberg, R. A. Stone, N. Kirshner, J. C. Gunnells, and R. R. Robinson
Plasma Dopamine beta-Hydroxylase: A Possible Aid in the Study and Evaluation of Hypertension
Science, February 8, 1974; 183(4124): 523 - 525.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Association for Clinical Chemistry.