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Clinical Chemistry 19: 103-105, 1973;
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Clinical Chemistry, Vol 19, 103-105, Copyright © 1973 by the American Association for Clinical Chemistry

AutoAnalyzer Assay for Serum Alkaline Phosphatase Activity, with Sodium Thymolphthalein Monophosphate as Substrate

Gary J. Proksch 1, Dean P. Bonderman 1, and John A. Griep 1

1 From the Department of Clinical Pathology, Indiana University Medical Center, Indianapolis, Ind. 46202

An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation

Submitted on May 29, 1972
Accepted on October 12, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.