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Clinical Chemistry 19: 118-120, 1973;
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Clinical Chemistry, Vol 19, 118-120, Copyright © 1973 by the American Association for Clinical Chemistry

Colorimetric Determination of 2,3-Diphosphoglycerate in Whole Blood

Anita V. Luisada-Opper 1

1 From the Division of Hepatic Metabolism and Nutrition, Department of Medicine, New Jersey Medical School of Newark, East Orange Division, 88 Ross St., East Orange, N.J. 07018

A method for determining 2,3-diphosphoglycerate (DPG) is described that has been adapted for the clinical laboratory and does not require any special equipment. For eight controls, the average value obtained by this method was 4.19 ± 0.26 µmol of DPG per milliliter of red blood cells. The procedure is simple, quick, and reliable


Key Words: phosphoglycerate mutase • Pi. determination after release from DPG

Submitted on July 28, 1972
Accepted on October 26, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.