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Clinical Chemistry 19: 125-126, 1973;
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Clinical Chemistry, Vol 19, 125-126, Copyright © 1973 by the American Association for Clinical Chemistry

Radioassay of Serum Folate, with Use of a Serum Blank and Nondialyzed Milk as Folate Binder

Mohammed Tajuddin 1 and Henry A. Gardyna 1

1 From the Boston Medical Laboratory, Inc., 15 Lunda St., Waltham, Mass. 02154

Serum folate determination by this method requires incubation of 0.1 ml of serum (or standards) with nondialyzed milk and then with tritium-labeled folate. A serum blank corrects for nonspecific binders present in the serum. Unbound folate is removed with hemoglobin-coated charcoal. The standard deviation of the results is ±0.2 µg/liter, and more than 90% of folate added to the serum is accounted for analytically. Serum folate concentrations as determined by this method agree with those obtained by microbiological assay (Lactobacillus casei), and our method is faster, simpler, and more accurate than other radioassay methods


Key Words: hemoglobin-coated charcoal as binder for folic acid • pteroylglutamate • microbiological assay compared • normal values • correction for nonspecific binders

Submitted on August 23, 1972
Accepted on October 30, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.