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Clinical Chemistry 19: 127-130, 1973;
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Clinical Chemistry, Vol 19, 127-130, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Manual and Automated Colorimetric Determination of Serum Glucose, with Use of Hexokinase and Glucose-6-Phosphate Dehydrogenase

Holly J. Coburn 1 and James J. Carroll 1

1 From the Diagnostics Research Department, Warner-Lambert Research Institute, 170 Tabor Road, Morris Plains, N. J. 07950

A previously reported assay for serum glucose, in which hexokinase and glucose-6-phosphate dehydrogenase were used, was improved to replace the light-sensitive phenazine methosulfate with "diaphorase," providing a one-reagent system. The assay system was automated for use with standard AutoAnalyzer equipment and evaluated at 40 and 60 samples per hour with a 2:1 sample-to-wash ratio. Sensitivity, precision, accuracy, and sample interaction were quite satisfactory at both sample rates. The assay benefits from the inherent advantages of the reaction mechanism: sensitivity, specificity, NADP regeneration, and freedom from dialysis


Key Words: single-reagent system for glucose • AutoAnalyzer • "diaphorase"

Submitted on October 6, 1972
Accepted on November 2, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.