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Clinical Chemistry 19: 27-30, 1973;
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Clinical Chemistry, Vol 19, 27-30, Copyright © 1973 by the American Association for Clinical Chemistry

The Use of Laboratory Computers in Monitoring Kinetic Enzyme Assays

G. Phillip Hicks 1, R. A. Ziesemer 1, and Norbert W. Tietz 1

1 From Laboratory Computing, Inc., Madison, Wis. 53716 (G.P.H. and R.A.Z.); Mount Sinai Hospital Medical Center and The University of Health Sciences, The Chicago Medical School, Chicago, Ill. 60608 (N.W.T.).

An online computer program to monitor kinetic enzyme assays is described. The program analyzes the kinetic data in a manner similar to the way a technologist handles data in a manual procedure, taking into account the lag phase, substrate depletion phase, and linear portion of the rate curve. Thus, complete automation of even complex kinetic assays has been made practical. The program has been implemented for routine use in a clinical laboratory computer system ("LABCOM"), and the results correlate well with those obtained by established methods of manual data-handling procedures


Key Words: LABCOM • KINTRAC VII • variations in enzyme reaction-rate curves • principle and operation of kinetic computer program • manual measurements compared

Submitted on August 7, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.