Clinical Chemistry
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Clinical Chemistry 19: 45-48, 1973;
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Clinical Chemistry, Vol 19, 45-48, Copyright © 1973 by the American Association for Clinical Chemistry

Simplified, Rapid Measurement of [131I] Triiodothyronine Uptake, with Use of Competitive Charcoal Adsorption

William R. Holub 1, Catherine F. Tortorella 1, and Madelaine R. Ruhl 1

1 From the Central Health Laboratories, Research Department, Health Services Division of USV Pharmaceutical Corp., 636 Wantagh Ave., Levittown, N. Y. 11756

Hemoglobin-coated charcoal has been used as a competitive binder in developing a [131I] triiodothyronine uptake assay. Modifying the procedures of Herbert et al. [J. Lab. Clin. Med. 66, 814 (1965)] and Braverman et al. [J. Amer. Med. Ass. 199, 469 (1967)] and examining various optimum test parameters, we developed a rapid, simpler technique. Correlation with results of the "Triosorb-131" resin sponge assay (Abbott Laboratories) was 0.909. Inter-run precision for a lyophilized serum control was satisfactory (CV, 2.5%). Advantages of the present method include: rapid equilibration time (10 min), easily prepared and dispensed adsorbent, nonquantitative transfer of final reaction mixture, and a linear response to variation in concentrations of binding protein that is of use in the diagnosis of thyroid dysfunction


Key Words: Hemoglobin-coated charcoal as competitive binder • resin sponge assay • normal values • T3 and T4 • hyper-and hypothyroidism

Submitted on July 14, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.