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Clinical Chemistry 19: 92-98, 1973;
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Clinical Chemistry, Vol 19, 92-98, Copyright © 1973 by the American Association for Clinical Chemistry

Increased Aspartate Aminotransferase Activity of Serum after in Vitro Supplementation with Pyridoxal Phosphate

Robert Rej 1, Charles F. Fasce Jr. 1, and Raymond E. Vanderlinde 1

1 From the New York State Health Department, Division of Laboratories & Research, Albany, N.Y. 12201

We examined the effect of pyridoxal phosphate supplementation on the apparent aspartate aminotransferase (EC 2.6.1.1.) activity of human serum. Supplementation by 25 µmol/liter effected an average increase of 16% in the results for kinetic assay. The increase was not the result of increased enzymatic or nonenzymatic blanks, and, within a small range, sample dilution had no significant effect. Part of the increase was attributable to the enzyme being protected against the loss of activity that occurs during preincubation with L-aspartate. A similar increase was not demonstrated in a two-point colorimetric method, perhaps because of the short reaction time, without preincubation, and the initial presence of both substrates in the assay. We attempted to Correlate such stimulation of aminotransferase activity and the patient’s diagnosis or treatment. Pyridoxal phosphate should be included in the reaction mixture when aspartate aminotransferase activity is being measured clinically


Key Words: differences in results by kinetic and colorimetric methods • serum enzyme activity

Submitted on October 18, 1972
Accepted on November 10, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.