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Clinical Chemistry, Vol 19, 1101-1105, Copyright © 1973 by the American Association for Clinical Chemistry
1 Patterson Coleman Laboratories, 4600 N. Habana,
Tampa, Fla. 33614.
We describe a radioassay procedure for serum folate, in which commercially available crystalline 
-lactoglobulin is used as the folate-binding agent. The
folate binders present in serum are eliminated by
heating serum in a boiling water bath for 15 min to
denature the proteins. Radioassayable serum folate
is shown to be stable under these conditions, and
the results for folate compare well with those obtained by a microbiological assay.
-lactoglobulin binding of folate • comparison with microbioassay • stability of folate • elimination of folate binding
by serum proteins • normal values
Submitted on May 18, 1973
Accepted on July 5, 1973
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