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Clinical Chemistry 19: 1112-1113, 1973;
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Clinical Chemistry, Vol 19, 1112-1113, Copyright © 1973 by the American Association for Clinical Chemistry

Rapid, Sensitive, and Selective Assay for Tryptophan in Serum

George G. Guilbault 1 and Peter M. Froehlich 1

1 Department of Chemistry, Louisiana State University in New Orleans, New Orleans, La. 70122.

We have developed a rapid, selective, and sensitive fluorometric procedure for measuring tryptophan in serum. This assay is based on the facile reaction of tryptophan with aqueous formaldehyde; the concentration of the amino acid is measured from the diminution in its fluorescence as the reaction proceeds. Compared to other assay procedures, this procedure has the advantage of being sensitive to the amino acid, but unresponsive to other indole derivatives such as tryptamine and 5-hydroxytryptamine. Concentrations of tryptophan as low as 0.5 mg/liter may be assayed in 15 min.


Key Words: monitoring dialysis of uremics • tryptophan fluorescence assay

Submitted on May 3, 1973
Accepted on July 9, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.