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Clinical Chemistry 19: 1122-1127, 1973;
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Clinical Chemistry, Vol 19, 1122-1127, Copyright © 1973 by the American Association for Clinical Chemistry

Computer-Controlled Instrument System for Sequential Clinical Chemical Testing. II. Evaluation of Instrumental Performance

E. Clifford Toren Jr. 1, Stephen A. Mohr 1, Michael G. Busby 1, and George S. Cembrowski 1

1 University of Wisconsin Center for Health Sciences, Departments of Medicine and Pathology, Madison, Wis. 53706.

The instrumental system described [Clin. Chem. 19, 1114 (1973)] is evaluated in manual, partially automated, and totally automated modes to measure system and individual component performance. Excellent accuracy and precision were observed, hence the system is judged suitable for most analytical applications. Results are: wavelength accuracy and reproducibility in automated mode: ±0.004 nm and ±0.1 nm, respectively; photometric accuracy and precision: ca. 0.8% relative and ±0.007 A, respectively, in both the manual and automated modes; and ratemeter accuracy and precision: ±0.4% relative and ±0.873 (SD) mV/min (or mA/ min), respectively, for standard synthetic ramps and ±1.2% relative and ±2.2 (SD) mV/min, respectively, under actual laboratory conditions for rates in the range of 1 to 200 mV/min. Automated experiments are made without human intervention after the samples are loaded.


Key Words: accuracy and precision • manual and automated modes of operation • evaluation of variables in the system • alkaline phosphatase

Submitted on May 25, 1973
Accepted on July 6, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.