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Clinical Chemistry 19: 1135-1138, 1973;
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Clinical Chemistry, Vol 19, 1135-1138, Copyright © 1973 by the American Association for Clinical Chemistry

Ammonium Thymolphthalein Monophosphate as a New Substrate for Alkaline and Acid Phosphatase Determinations in Serum

Leo G. Morin 1

1 Chemistry Department, Research Section, Kiess Instruments, Inc., 8768 Southwest 131st St., Miami, Fla. 33156.

A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at pH 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 min, and at pH 5.9 in citrate buffer for determining acid phosphatase activity in 30 min. The substrate is relatively specific for bone and liver alkaline phosphatase and prostatic acid phosphatase. Coefficients of variation were 2.5% and 6.6% for alkaline and acid phosphatase, respectively.

Submitted on June 21, 1973
Accepted on July 24, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.