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Clinical Chemistry 19: 1139-1141, 1973;
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Clinical Chemistry, Vol 19, 1139-1141, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Techniques for Assessment of Serum Lipoprotein Patterns. II. Rapid Method for Diagnosis of Type III Hyperlipoproteinemia without Ultracentrifugation

Heinrich Wieland 1 and Dietrich Seidel 1

1 Medizinische Universitätsklinik (Ludolf-Krehl-Klinik), Bergheimer Strasse 58, D-69 Heidelberg, F. R. G.

Based on the previously described technique [Clin. Chem. 19, 737 (1973)] of precipitating plasma lipoproteins with polyanions after their electrophoretic separation in gels, a new method is presented for diagnosing type III hyperlipoproteinemia without need for ultracentrifugation or immunologic techniques. Used in the procedure are 0.1 mol/liter MgCl2, 1.5 g/liter heparin, and 10 g/liter NaCl to visualize selectively the very-low-density lipoproteins in agarose gel after electrophoresis. The technique is simple, inexpensive, accessible to every laboratory, and provides the answer in less than 2 h after electrophoresis of the patient’s whole serum. The results obtained are the same as those obtained by ultracentrifugation followed by lipoprotein electrophoresis of the isolated fractions.

Submitted on May 8, 1973
Accepted on July 24, 1973




The following articles in journals at HighWire Press have cited this article:


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 J. Lipid Res.Home page
D. J. Blom, P. Byrnes, S. Jones, and A. D. Marais
Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia
J. Lipid Res., January 1, 2003; 44(1): 212 - 217.
[Abstract] [Full Text] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.