Clinical Chemistry
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Clinical Chemistry 19: 1148-1151, 1973;
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Clinical Chemistry, Vol 19, 1148-1151, Copyright © 1973 by the American Association for Clinical Chemistry

A Turbidimetric Continuous-Flow System for Immunochemical Quantitation of Blood Proteins by Use of a Modified Photometer

Mogens Blom 1 and Darly Sørensen 1

1 Department of Clinical Chemistry, Aalborg Hospital South, 9000 Aalborg, Denmark.

In a continuous-flow procedure, the turbidity resulting from the reaction between albumin and transferrin and specific anti-human sera has been measured in a photometer constructed for the purpose. Incorporation of two photoelectric cells and a special bridge built between the photometer and the recorder made possible the amplification of the sensitivity by as much as 30-fold. Further, a more economical intermittent flow of antiserum was used. The standard deviations for sera analyzed in duplicate (day-to-day) were 1.4 and 0.05 g/liter, respectively, for albumin and transferrin determinations. Values obtained by this method correlated well with values obtained by the electroimmunotechnique.


Key Words: determination of albumin, transferrin • electroimmunoassay compared

Submitted on April 2, 1973
Accepted on August 2, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.