Clinical Chemistry, Vol 19, 1152-1157, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Glucose Methods: Evaluation of a Glucose Oxidase—Peroxidase Procedure

¹ 331 Willow Avenue, Hoboken, N. J. 07030.

We have evaluated methods in which glucose oxidase is used to determine serum glucose with the "AutoAnalyzer-II" and "SMA 12/60" (Technicon). A glucose oxidase—peroxidase—dianisidine procedure with improved specificity developed during this study is described. The glucose value is unaffected by the presence of 16 mg of uric acid per deciliter, and the system is highly resistant to interference from many other reducing compounds added to serum. The method appears to give "true glucose" values for normal, hyperuricemic, and uremic subjects. Results are compared with those from the "GOD-Perid," glucose oxidase—peroxidase—MBTH—DMA, hexokinase, toluidine, ferricyanide, and SMA 12/60 neocuproine techniques, as well as with those from our index of "true glucose," a procedure based on measurement of reducing material before and after treatment with glucose oxidase. Specificity and performance of the glucose oxidase—peroxidase—dianisidine and glucose oxidase—peroxidase—MBTH—DMA procedures are similar.

Submitted on June 13, 1973
Accepted on August 8, 1973

The following articles in journals at HighWire Press have cited this article:

				R. H. Knopp, C. E. Walden, B. M. Retzlaff, B. S. McCann, A. A. Dowdy, J. J. Albers, G. O. Gey, and M. N. Cooper Long-term Cholesterol-Lowering Effects of 4 Fat-Restricted Diets in Hypercholesterolemic and Combined Hyperlipidemic Men: The Dietary Alternatives Study JAMA, November 12, 1997; 278(18): 1509 - 1515. [Abstract] [PDF]

				M. S. Magee, C. E. Walden, T. J. Benedetti, and R. H. Knopp Influence of Diagnostic Criteria on the Incidence of Gestational Diabetes and Perinatal Morbidity JAMA, February 3, 1993; 269(5): 609 - 615. [Abstract] [PDF]