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Clinical Chemistry 19: 1162-1169, 1973;
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Clinical Chemistry, Vol 19, 1162-1169, Copyright © 1973 by the American Association for Clinical Chemistry

Rapid Determination of Ammonia in a Perchloric Acid Supernate from Blood, by Use of an Ammonia-Specific Electrode

Henning F. Proelss 1 and Billy W. Wright 1

1 Baptist Medical Center, 701 Princeton Ave., S. W., Birmingham, Ala. 35211.

A simple, rapid method was developed for accurate determination of ammonia in whole blood. Blood proteins were precipitated with perchloric acid (8 g/dl) and the free ammonia liberated in the supernate on alkalinization was measured directly with an ammonia-specific electrode after adjusting the sample temperature to 25 °C. Some variables affecting precision, accuracy, and electrode performance were studied. Sensitivity, specificity, and interferences are discussed. The tentative normal range is 28 ± 14 µg of ammonia nitrogen per deciliter. The coefficient of variation was 4.8% in the "normal," 3.6% in the "abnormal" range. Abnormal values were correlated with clinical status. Recoveries averaged 99.3%. Correlation with an established ion-exchange method for plasma ammonia was 0.994. Total time required for a complete assay is 15 min.


Key Words: precision and accuracy, variables affecting • normal values • glutamine hydrolysis • labile amides in blood • liver disease

Submitted on July 11, 1973
Accepted on August 14, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.