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Clinical Chemistry 19: 1179-1183, 1973;
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Clinical Chemistry, Vol 19, 1179-1183, Copyright © 1973 by the American Association for Clinical Chemistry

Urinary Proteins Studied by Use of Isoelectric Focusing. I. Tubular Malfunction in Association with Exposure to Cadmium

O. Vesterberg 1 and G. Nise 1

1 Chemical Division, Occupational Health Department, The National Board of Occupational Safety and Health, S-10026 Stockholm 34, Sweden.

Isoelectric focusing in thin-layer polyacrylamide gel has been applied to studies of urinary proteins. We could differentiate the following patterns: tubular, glomerular with high and low selectivity, as well as mixed glomerular and tubular patterns, as seen in some cases of chronic pyelonephritis and uremia. The typical tubular-protein patterns—i.e., as developed in association with cadmium exposure—were characterized by elevated excretion of beta2-microglobulin. Possibly the severity of renal malfunction can be assessed by the isoelectric focusing technique. To identify the protein zones, we in some cases complemented isoelectric focusing with electrophoresis at right angles in acrylamide gel and also in agarose gel containing antibodies to provide additional information.


Key Words: diagnostic acid • beta2-microglobulin • environmental hazards • renal disease, tubular and glomerular

Submitted on March 27, 1973
Accepted on August 17, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.