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Clinical Chemistry 19: 1187-1192, 1973;
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Clinical Chemistry, Vol 19, 1187-1192, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Method for Determining Amylase Activity in Serum and Urine

A. Mazzuchin 1, C. Weggel 1, and C. J. Porter 1

1 Toronto General Hospital, Department of Clinical Biochemistry, Toronto, Ontario, Canada.

We describe an automated procedure for determination of agr-amylase activity in serum or urine by use of a water-insoluble chromogenic starch substrate (commercially available). "AutoAnalyzer" (Technicon Corp.) equipment is used in which the sample and suspended substrate are introduced simultaneously into the flow system. After incubation at 37 °C the water-soluble blue-starch breakdown products are filtered on-line across a cellulose nitrate membrane. Absorbance is measured at 630 nm, and the agr-amylase activity read from a standard curve. The automated procedure has been in use for the past year and has been found to be an operationally simple, reproducible, and reliable method, which can be applied over a wide range of enzyme activity. Results of comparative studies with other agr-amylase methods are also described.


Key Words: "Phadebas Amylase Kit" • AutoAnalyzer • normal range

Submitted on June 14, 1973
Accepted on August 17, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.