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Clinical Chemistry 19: 1193-1195, 1973;
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Clinical Chemistry, Vol 19, 1193-1195, Copyright © 1973 by the American Association for Clinical Chemistry

Assessment of the Need for Triglyceride Blank Measurements

Gopal S. Rautela 1, Suzanne Slater 1, and Dean A. Arvan 1

1 William Pepper Laboratory, Hospital of the University of Pennsylvania, 3400 Spruce St., Philadelphia, Pa. 19104.

Two methods of triglyceride blank measurement were critically investigated. For 38 samples a mean blank value of 7.2 mg/dl was obtained when the blanks were measured by replacing the saponifying reagent with 80% isopropanol. For the same samples, consistently higher blank values were obtained (mean = 18.3 mg/dl) when these were determined by the Kessler-Lederer method. It is suggested that, when true blank values are desired, the former method is to be preferred. Based on theoretical considerations, however, as well as on our experimental data on the effect of in vitro hydrolysis on triglyceride blank measurements, we recommend that in routine triglyceride measurements, blank determinations are not needed.


Key Words: spontaneous hydrolysis • Hantzsch’s condensation reaction

Submitted on May 24, 1973
Accepted on July 24, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.