Clinical Chemistry
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Clinical Chemistry 19: 1204-1206, 1973;
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Clinical Chemistry, Vol 19, 1204-1206, Copyright © 1973 by the American Association for Clinical Chemistry

Use of "Sephadex G-15" in an Improved Method for Estrogens in Pregnancy Urine

Evelyn S. C. Quek 1, J. E. Buttery 1, and G. F. de Witt 1

1 Division of Biochemistry, Institute for Medical Research, Kuala Lumpur 02-14, Malaysia.

A simple, rapid, and reliable colorimetric method has been developed for determining total estrogens in the urine of pregnant women. It is essentially a modification of the procedure of Van Baelen et al. [J. Clin. Endocrinol. 27, 1056 (1967)], in which gel adsorption is used as a purification step after enzymatic hydrolysis. Three major modifications have been made to the original procedure, whereby a single determination can be completed within 4 h. Actual manipulative time of the analysis is only 2 h, and one technician can easily handle an average of 10 cases per batch. Precision of the method is good, the coefficient of variation being less than 5%. Recovery of added estriol is better than 95%. The method can be used for measuring estrogen excretions ranging from 0.5-50 mg/24 h. We have found it very suitable as a routine assay in our laboratory.


Key Words: adsorption on gel • Kober reaction • monitoring high-risk pregnancies

Submitted on June 6, 1973
Accepted on August 6, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.