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Clinical Chemistry 19: 1208-1213, 1973;
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Clinical Chemistry, Vol 19, 1208-1213, Copyright © 1973 by the American Association for Clinical Chemistry

A Referee Method for the Determination of Total Calcium in Serum

J. Paul Cali 1, G. N. Bowers Jr. 2, and D. S. Young 3

1 Institute for Materials Research, National Bureau of Standards, Washington, D.C. 20234
2 Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115
3 Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Md. 20014

The first referee method of analysis (a method of known accuracy) in clinical chemistry has been developed through the cooperation of scientists from the National Bureau of Standards and eight clinical chemistry laboratories. Subject to some limitations discussed in the text, calcium in serum can be determined by the referee method to within ±2% of the "true or absolute" value. The complete protocol, based on an atomic absorption spectrometric procedure, is described in detail.




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