Clinical Chemistry
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Clinical Chemistry 19: 1259-1264, 1973;
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Clinical Chemistry, Vol 19, 1259-1264, Copyright © 1973 by the American Association for Clinical Chemistry

Design and Evaluation of a Filter Fluorometer That Incorporates a Photon-Counting Detector

Robert E. Curry 1, Harry L. Pardue 1, Glen E. Mieling 1, and Robert E. Santini 1

1 Department of Chemistry, Purdue University, Lafayette, Ind. 47907.

We describe the design and evaluation of a filter fluorometer in which a photomultiplier detector is used in the photon-counting mode. Output frequency is shown to be linear with light intensity from 5 Hz to 800 kHz. Comparisons with commercially available instruments show an inherent sensitivity superior to a commercially available filter instrument and comparable to that of a spectrofluorometer. Results are included showing the applicability of the instrument to the kinetic determination of lactate dehydrogenase in serum.


Key Words: lactate dehydrogenase • fluorescein • rhodamine B • improved sensitivity

Submitted on April 30, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.