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Clinical Chemistry 19: 1276-1279, 1973;
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Clinical Chemistry, Vol 19, 1276-1279, Copyright © 1973 by the American Association for Clinical Chemistry

Bilirubin Binding Affinity of Serum: Comparison of Qualitative and Quantitative Sephadex Gel Filtration Methods

N. A. Kaufmann 1, J. Kapitulnik 1, and S. H. Blondheim 1

1 Metabolic Laboratory and Departments of Medicine B and Nutrition, Hadassah—University Hospital and Hebrew University—Hadassah Medical School, Jerusalem, Israel.

A simple, 30-min method of estimating the binding of bilirubin to serum protein was compared with a quantitative method. Good correlation was found between the two methods, both based on Sephadex gel filtration. The results indicate that the presence of loosely bound bilirubin can be determined with this simple, qualitative, semimicro-scale test. This determination is important for the clinical evaluation of jaundiced infants, since it may help to determine whether and when prophylactic therapy to prevent bilirubin encephalopathy is appropriate.


Key Words: neonatal jaundice • bilirubin encephalopathy • diagnostic aid • "Ictotest" • brain damage • kernicterus

Submitted on August 9, 1973




The following articles in journals at HighWire Press have cited this article:


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 PediatricsHome page
R. P. Wennberg, C. E. Ahlfors, V. K. Bhutani, L. H. Johnson, and S. M. Shapiro
Toward Understanding Kernicterus: A Challenge to Improve the Management of Jaundiced Newborns
Pediatrics, February 1, 2006; 117(2): 474 - 485.
[Abstract] [Full Text] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.