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Clinical Chemistry 19: 1280-1284, 1973;
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Clinical Chemistry, Vol 19, 1280-1284, Copyright © 1973 by the American Association for Clinical Chemistry

Residual Fluorescence as an Index of Purity of Reduced Nicotinamide Adenine Dinucleotide

Barbara F. Howell 1, Sam Margolis 1, and Robert Schaffer 1

1 Analytical Chemistry Division, National Bureau of Standards, Washington, D. C. 20234.

Determination of fluorescence remaining after reduced nicotinamide adenine dinucleotide (NADH) has reacted with excess acetaldehyde in the presence of alcohol dehydrogenase (EC 1.1.1.1) is useful as a criterion of NADH purity when used in conjunction with other methods for determining purity such as the rate of reaction, the ratio of ultraviolet absorbances at 260 nm and 340 nm, the color, and the chromatographic homogeneity of the preparation. Measurement of residual fluorescence monitors the enzymatically inactive material which absorbs at 340 nm. The specific optical rotations of NADH at several wavelengths are also reported.


Key Words: specific optical rotation of NADH

Submitted on July 25, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.