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Clinical Chemistry 19: 1285-1287, 1973;
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Clinical Chemistry, Vol 19, 1285-1287, Copyright © 1973 by the American Association for Clinical Chemistry

Improved Automated Determination of Bound N-Acetylneuraminic Acid in Serum

Lucile Gerbaut 1, Elisabeth Rey 1, and Christian Lombart 1

1 Laboratoire de Biochimie, Hôpital Saint-Vincent-de-Paul, 74, Avenue Denfert-Rochereau, 75014 Paris, France (L.G. and E.R.); and the Laboratoire de Biochimie, U.E.R. Biomédicale des Saints-Pères, 45, rue des Saints-Pères, 75270 Paris Cedex 06, France (C.L.).

The method in which thiobarbituric acid is used for sialic acid determination was adapted to a fully automated procedure, in which the chromogen need not be extracted with an organic solvent. The entire procedure, including hydrolysis, requires 35 min and 25 µl of serum for one analysis, but 30 samples can be analyzed per hour. The procedure, slightly modified, can be used to measure as little as 10 mg of bound sialic acid per liter.


Key Words: monitoring sialoglycoprotein changes in serum • "acute-phase reactant" proteins • AutoAnalyzer • thiobarbiturate reagent for sialic acids

Submitted on December 27, 1972






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Copyright © 1973 by the American Association for Clinical Chemistry.