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Clinical Chemistry 19: 1293-1295, 1973;
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Clinical Chemistry, Vol 19, 1293-1295, Copyright © 1973 by the American Association for Clinical Chemistry

Nitrile-Terminated Hydrocarbons as Stationary Phases for High-Performance Liquid Chromatography of Steroid Hormones

Francis A. Fitzpatrick 1

1 Dept. of Chemistry, University of Massachusetts, Amherst, Mass. 01002.

A high-performance liquid chromatographic method for separating steroid hormones by using nitrile-terminated hydrocarbons as the stationary phase is described. Particular selectivity toward the separation of corticosteroids with an agr, beta unsaturated ketone group is noted, with five steroids more polar than cortisol being completely resolved. The system described is also applicable to estrogen separations.

Submitted on July 25, 1973
Accepted on August 30, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.