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Clinical Chemistry 19: 1296-1299, 1973;
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Clinical Chemistry, Vol 19, 1296-1299, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Fluorometric Micromethod for Blood Glucose

Carl W. Seiter 1, Hoyle D. Hill Jr. 1, and George K. Summer 1

1 Department of Biochemistry and the Biological Sciences Research Center, Child Development Institute, School of Medicine, University of North Carolina, Chapel Hill, N. C. 27514.

G.K.S. at the Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill, N. C. 27514.

We describe a sensitive automated fluorometric method for glucose in whole blood spotted on filter paper. By action of glucose oxidase (EC 1.1.3.4) on glucose, hydrogen peroxide is produced, which then reacts with 4-hydroxy-3-methoxy-phenylacetic acid (homovanillic acid) in the presence of horseradish peroxidase (EC 1.11.1.7) to form a fluorescent condensation product. Obtained at a rate of 60 samples per hour, the results are accurate, linear, and reproducible. Whole blood glucose can be determined by this method with an error (CV) of less than 5%.


Key Words: homovanillic acid reagent for glucose • glucose in whole blood of newborns • AutoAnalyzer • loss on storage as dried blood • glucose tolerance curve

Submitted on June 4, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.