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Clinical Chemistry 19: 1302-1303, 1973;
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Clinical Chemistry, Vol 19, 1302-1303, Copyright © 1973 by the American Association for Clinical Chemistry

Preparation of Amine Salts of Thymolphthalein Monophosphate and Their Use for Measuring Phosphatase Activity

John M. Erikson 1 and Homer G. Biggs 1

1 Department of Clinical Pathology, Indiana University School of Medicine, Indianapolis, Ind. 46202.

An easy and quick preparation of amine salts of thymolphthalein monophosphate is described. All of the salts prepared are stable, nonhygroscopic, soluble powders. The 2-amino-2-methyl-1,3-propandiol salt is preferred for use as a substrate for measuring phosphatase activities because of its high solubility and easy preparation.

Submitted on June 9, 1973
Accepted on August 2, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.