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Clinical Chemistry 19: 1305-1306, 1973;
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Clinical Chemistry, Vol 19, 1305-1306, Copyright © 1973 by the American Association for Clinical Chemistry

Ferric Chloride-Impregnated Silica Gel for Thin-Layer Chromatography of Steroids

Daniel R. Shapiro 1 and Steven S. Kuwahara 1

1 Department of Developmental and Cell Biology, University of California, Irvine, Calif. 92664.

Silica gel G is mixed with an aqueous solution of ferric chloride, and the resulting slurry spread onto glass plates. After spotting, development, and drying, the plates are sprayed with sulfuric acid. Cholesterol, ergosterol, and Dgr5-pregnen-3beta-ol-20-one produce colored spots (pink-violet, greenish black, and pink-violet, respectively) without further treatment, but some other steroids require heating at 70 °C for full color development. Other colors generated are: estrone, orange; Dgr4-androsten-3,17-dione, blue-green; androsterone, light brown; and 5agr-androstan-17beta-ol-3-one, tan. After prolonged heating at 70 °C, 6-keto-cholestanol produces an orange-brown spot. Color reactions are more reproducible than they are when the ferric chloride is sprayed on the plate.

Submitted on July 30, 1973
Accepted on August 24, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.