Clinical Chemistry
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Clinical Chemistry 19: 1345-1349, 1973;
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Clinical Chemistry, Vol 19, 1345-1349, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Assay of Hexosaminidases in Serum

J. A. Lowden 1, M. A. Skomorowski 1, F. Henderson 1, and M. Kaback 1

1 The Research Institute, The Hospital for Sick Children, Toronto, Canada.

In serum there are two classes of hexosaminidase. One of these is heat-labile and is inactive in children with Tay—Sachs disease. Serum from heterozygotes for Tay—Sachs disease has half the normal activity of the heat-labile enzyme. The heat-labile enzyme can be inactivated by heating serum diluted in buffer for 5 min at 60 °C. We have used this property to develop a reliable, fully automated assay for serum hexosaminidases, which can be used for mass carrier screening programs.


Key Words: Tay—Sachs disease • screening technique • AutoAnalyzer • oral contraceptives • manual and automated methods compared

Submitted on June 29, 1973




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[Abstract] [Full Text] [PDF]


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Copyright © 1973 by the American Association for Clinical Chemistry.