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Clinical Chemistry 19: 1357-1360, 1973;
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Clinical Chemistry, Vol 19, 1357-1360, Copyright © 1973 by the American Association for Clinical Chemistry

Comparison of Two Methods for Radioassay of Vitamin B12 in Serum

Eugene P. Frenkel 1, Jerry D. White 1, Joan S. Reisch 1, and Richard G. Sheehan 1

1 E. L. Overton Hematology-Oncology Research Laboratory, Departments of Internal Medicine and Biostatistics, University of Texas Southwestern Medical School at Dallas, and Nuclear Medicine Service, VA Hospital, Dallas, Tex, 75235.

A reference radioassay for vitamin B12 in serum, in which human serum is used as binder and DEAE-cellulose is used to separate free and bound B12, was compared (194 sera) with a packaged kit (" Phadebas B12 Test"), in which intrinsic factor is used as binder and polysaccharide for separation. The correlation coefficient was 0.949. With the kit, added B12 was identifiable, proportional dilution characteristics were excellent, and serial reproducibility was good. The normal range for the reference assay was 200-1000 pg/ml; all B12-deficient patients had values of <165 pg/ml. In a separate study, 38 patients with documented (clinical and laboratory) B12 deficiency had mean serum values of <150 pg/ml by the reference assay, 250 pg/ml by the kit. For the kit, the normal range was 250-1100 pg/ml. All patients with serum values of <150 pg/ml by kit assay were B12 deficient; false positives were seen in the 150-250 pg/mI range.


Key Words: normal range • commercial kit • " Sephadex" • " Phadebas B12 Test" • anemia • diagnostic aids

Submitted on July 23, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.