Clinical Chemistry
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Clinical Chemistry 19: 1361-1365, 1973;
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Clinical Chemistry, Vol 19, 1361-1365, Copyright © 1973 by the American Association for Clinical Chemistry

Solid-Phase Radioimmunoassay for Digoxin

William F. Line 1, Stanley J. Siegel 1, Anda Kwong 1, Clifford Frank 1, and Roberta Ernst 1

1 Research Department, The Wilson Laboratories, 2600 Bond St., Park Forest South, Ill. 60466.

Anti-digoxin serum was conjugated to agarose or to organo-functional glass beads. The immunoadsorbents were compared to each other and to the soluble antibody, with respect to coupling efficiency and retention of immunological activity. We found that the antisera coupled to a glass-isothiocyanate retained about 50% of its immunological activity upon attachment. This derivative was superior to one prepared by coupling to CNBr-activated agarose, in which the attached protein retained 7% of its activity; and one prepared by coupling the protein to alkylamino glass using a water soluble carbodiimide, in which the attached antiserum retained only 1.5% of its original activity.


Key Words: conjugation of antiserum to agarose or organo-functional glass beads vs. conjugation to CNBr-activated agarose or glass-isothiocyanate

Submitted on August 17, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.