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Clinical Chemistry 19: 1366-1369, 1973;
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Clinical Chemistry, Vol 19, 1366-1369, Copyright © 1973 by the American Association for Clinical Chemistry

Single-Reagent Method for Rapid Determination of Total Bilirubin with the "CentrifiChem" Analyzer

Gerhard Ertingshausen 1, Diane L. Fabiny Byrd 1, Thomas O. Tiffany 1, and Sandra J. Casey 1

1 Union Carbide Research Institute, Tarrytown, N. Y. 10591 (G. E. and D. L. F. B.); and the Department of Clinical Biochemistry, E. J. Meyer Hospital Division, Erie County Laboratories, Buffalo, N. Y. 14215.

We report a rapid single-reagent method for determining total bilirubin with the " CentrifiChem Analyzer" (Union Carbide Corp.). The reagent is the stable 1,5-disulfonate of diazosulfanilic acid, and ethylene glycol is used as an accelerator. The reaction requires less than 5 min. Only 25 µl of serum is needed. We present precision data (x = 2.62 mg/dl, SD = .082 mg/dl, CV = 3.13%) and results (r = .998) as compared with those obtained by an automated Jendrassik—Grof method.


Key Words: stabilized diazonium salt of sulfanilic acid • ethylene glycol as accelerator • interference by indican • AutoAnalyzer • Jendrassik—Grof procedure compared

Submitted on June 11, 1973




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. M. Henrich, D. E. Bruns, D. M. Haverstick, V. G. Reynolds, and J. C. Boyd
Indican Interference in Bilirubin Assays: A Classical Solution Still Applies
Clin. Chem., February 1, 2007; 53(2): 359 - 361.
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Copyright © 1973 by the American Association for Clinical Chemistry.