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Clinical Chemistry 19: 1370-1373, 1973;
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Clinical Chemistry, Vol 19, 1370-1373, Copyright © 1973 by the American Association for Clinical Chemistry

Use of Horseradish Peroxidase in Identification of Serum Antibodies and Immune Complexes

William M. Murphy 1, Sharad D. Deodhar 1, and Leo P. Cawley 1

1 Department of Immunology, Cleveland Clinic, Cleveland, Ohio; and the Department of Laboratory Medicine, Wesley Medical Center, Wichita, Kan.

We made 202 examinations on specimens from 78 patients, to evaluate the application of horseradish peroxidase-labeled antisera as compared to fluorescein-labeled material in a clinical immunology laboratory. Various circulating and tissue-fixed antibodies were identified by both techniques. The horseradish peroxidase technique can be substituted for the fluorescein isothiocyanate method for the evaluation of anti-nuclear antibody and anti-mitochondrial antibody without loss of specificity or sensitivity. Although the results are comparable, fluorescein isothiocyanate appears to be slightly more sensitive in the detection of the other circulating and tissue-fixed human antibodies examined. Further refinements in preparation and technique could well lead to the fluorescein method being replaced by horseradish peroxidase staining in the future.


Key Words: diagnosis of autoimmune diseases • fluorescein isothiocyanate method compared

Submitted on July 10, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.