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Clinical Chemistry 19: 1394-1396, 1973;
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Clinical Chemistry, Vol 19, 1394-1396, Copyright © 1973 by the American Association for Clinical Chemistry

Simple, Rapid Determination of Lecithin and Sphingomyelin in Amniotic Fluid

K. G. Blass 1, R. J. Thibert 1, and T. F. Draisey 1

1 Department of Chemistry, University of Windsor, Windsor, Ontario, Canada N9B 3P4; and The Salvation Army Grace Hospital, University and Crawford Ave., Windsor, Ontario, Canada N9A 5C6.

We describe a rapid and very sensitive modified determination of lecithin and sphingomyelin in amniotic fluid. It requires 1.5 ml of amniotic fluid and can be done in less than 40 min. Modifications include "Instant Thin Layer Chromatography" plates (Gelman) with a punched disc system of sample application, as well as elimination of the acetone wash steps, and substitution of Rhodamine B dye for the corrosive sulfuric acid char technique of detection. The procedure can be quantified, and exact concentrations of lecithin in amniotic fluid can be determined by a standard addition procedure, or from a standard curve.


Key Words: thin-layer chromatography • respiratory distress syndrome • L/S ratio

Submitted on July 23, 1973
Accepted on October 5, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.