Clinical Chemistry
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Clinical Chemistry 19: 146-186, 1973;
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Clinical Chemistry, Vol 19, 146-186, Copyright © 1973 by the American Association for Clinical Chemistry

Radioimmunoassay

D. S. Skelley 1, L. P. Brown 1, and P. K. Besch 1

1 Department of Obstetrics and Gynecology, Baylor College of Medicine, and The Reproductive Research Laboratory, St. Luke’s Episcopal Hospital, Texas Medical Center, Houston, Texas 77025.

Principles of radioimmunoassay are discussed. Methods are outlined for preparing radiolabeled antigenic compounds and characterizing antibodies. The various techniques for separating bound and free antigen are also reviewed. Commercial suppliers of various components needed for radioimmunoassay are listed. Also presented is a selected list of normal values for hormonal and nonhormonal substances from humans.


Key Words: competitive protein-binding analysis • immunoradiometric assay • cross-reactivity • antigenic radiolabeling • antibody induction • separation techniques • analytical considerations • normal human values • technical variations of RIA




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Copyright © 1973 by the American Association for Clinical Chemistry.